gold anti fade reagent with dapi  (Beyotime)

Journal: Bioactive Materials

Article Title: Sulfated polysaccharide facilitates macrophage-Treg crosstalk to mitigate chronic inflammation in diabetic wound healing

doi: 10.1016/j.bioactmat.2025.09.044

Figure Lengend Snippet: GelMa/SCS-MN promotes re-epithelialization and revascularization in diabetic wounds. (a) Photographs of wounds of diabetic mice at different time after treatments. (b) Quantitative analysis of the relative residual wound areas at different times (n = 6). (c) Sagittal sections of day 3 and day 7 wound tissue immunolabeled for cytokeratin (red), integrin a5 (Itga5, green) and DAPI (blue). Scale bar, 200 μm. (d) Sagittal sections of day 7 wound tissue immunolabeled for cytokeratin (red), Itga5 (green) and labeled with 5-ethynyl-2′-deoxyuridine (EdU, gray) (proliferation), cell nucleus for DAPI (blue). Scale bar, 100 μm. (e) Quantitative statistics of the migration distance of epidermal tongue from wound edge (n = 5). (f) Quantitative statistics of the number of EdU + epidermal cells in each visual field (n = 5). (g) Sagittal sections of day7 wounds tissue immunolabeled for Endomucin (Emcn, red) and EdU (green), cell nucleus for DAPI (blue). Scale bar, 200 μm. (h) Quantitative statistics of the Emcn + vascular gap in wound (n = 5). (i) Representative H&E staining of diabetic wound tissue on day7 and day 14. The green line indicates epidermal gap. Scale bar, 200 μm. (j–k) Quantification of (j) epidermis gap on day 7 and (k) scar distance on day 7 and day 14 according to HE staining images (n = 3). (l) Sagittal sections of day14 wounds tissue immunolabeled for Emcn (red), cell nucleus for DAPI (blue). Scale bar, 200 μm. Data are presented as mean ± SD and statistical significance was analyzed via one-way ANOVA with Tukey's multiple comparison test for (f), (h) and (j), two-way ANOVA with Sidak's multiple comparison test for (b), (e), and (k). P value: ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.

Article Snippet: The specimens were sectioned to a thickness of 10 μm, washed three times with TBST, and mounted using ProLong gold anti-fade reagent with DAPI (Cell Signaling Technology).

Techniques: Immunolabeling, Labeling, Migration, Staining, Comparison

Journal: Bioactive Materials

Article Title: Sulfated polysaccharide facilitates macrophage-Treg crosstalk to mitigate chronic inflammation in diabetic wound healing

doi: 10.1016/j.bioactmat.2025.09.044

Figure Lengend Snippet: Lacking Tregs impairs SCS-mediated diabetic wound healing. (a) Schematic illustrating experiment depleting Tregs. (b) Flow cytometric quantification of Tregs (Foxp3 + ) in the diabetic skin wound after anti-CD25 mAb injection (n = 6). (c) Representative wound images of diabetic mice after the indicated treatments and the corresponding time-course quantification of relative residual wound area (n = 8). (d) Sagittal sections of day 7 wound tissue immunolabeled for cytokeratin (red), integrin a5 (Itga5) (green), EdU (gray) and DAPI (blue). Scale bar, 200 μm. (e) Quantitative statistics of the migration distance of epidermal tongue from wound edge (n = 8). (f) Quantitative statistics of the number of EdU + epidermal cells in each visual field (n = 12). (g) Representative flow cytometric plots of CD31 + Emcn + cells expression in diabetic wound tissue on day7 post-wounding and corresponding quantitative results (n = 6). (h) Representative immunostainings of CD31 (red), Emcn (green), EdU (gray) and DAPI (blue) in diabetic wound tissue on day7 post-wounding. Scale bar, 200 μm. (i) Representative H&E staining of diabetic wound tissue on day7 and day14. Scale bar, 500 μm. (j–k) Quantification of (j) epidermis gap on day 7 and (k) scar distance on day14 according to H&E staining images (n = 3). Data are presented as mean ± SD and statistical significance was analyzed via two-way ANOVA with Sidak's multiple comparison test for (c), unpaired two-tailed Student's t -test for (b), (e), (f), (g), (j) and (k). P value: ∗P < 0.05, ∗∗P < 0.01.

Article Snippet: The specimens were sectioned to a thickness of 10 μm, washed three times with TBST, and mounted using ProLong gold anti-fade reagent with DAPI (Cell Signaling Technology).

Techniques: Injection, Immunolabeling, Migration, Expressing, Staining, Comparison, Two Tailed Test